Skyline is a freely-available, open-source Windows client application for building Selected Reaction Monitoring (SRM) / Multiple Reaction Monitoring (MRM), Parallel Reaction Monitoring (PRM), DIA/SWATH and targeted DDA quantitative methods and analyzing the resulting mass spectrometer data. Its flexible configuration supports All Molecules. It aims to employ cutting-edge technologies for creating and iteratively refining targeted methods for large-scale quantitative mass spectrometry studies in life sciences.

Researchers should cite this work as follows:

• (2024), "Skyline," https://sciencegateways.org/resources/skyline.

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1. DDA quantitative methods
2. MacCoss Lab Software
3. Molecules
4. Panorama
5. Skyline
6. softwareEntry
7. spectrometer data