Protein Crystallisation Construct Designer (ProteinCCD)
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Abstract
The Protein Crystallisation Construct Designer (ProteinCCD) is a tool to help deciding how to choose promising constructs for protein expression and crystallisation. A specific feature of CCD is that since it starts from the DNA sequence, it keeps track of the protein-DNA relationship. Thus, although all the analysis and construct choice is being done in the protein level, CCD can be used to suggest primers for PCR amplification of the chosen constructs, since it also knows the DNA sequence.
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ProteinCCD: enabling the design of protein truncation constructs for expression and crystallization experiments Wijnand T. M. Mooij, Eirini Mitsiki and Anastassis Perrakis Nucl. Acids Res. (2009) 37 (suppl 2): W402-W405. DOI: 10.1093/nar/gkp256
a.perrakis@nki.nl
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